生物素-PEG3-疊氮化物是一種不可裂解的疊氮化物活化生物素化試劑，通過銅催化的點擊反應與末端炔烴反應反應，通過無銅點擊反應與應變的環辛炔（例如DBCO或BCN化合物）反應，或通過稱為Staudinger化學的機制與磷化氫標記的分子反應，從而實現生物樣品中衍生化分子的高效和特異性偶聯。這些反應是生物正交的，或者換句話說，與生物系統相容，因為它的成分不與生物環境發生反應。

Biotin-PEG3-Azide is non-cleavable, azide-activated biotinylation reagent that reacts with terminal alkynes via a copper-catalyzed click reaction, strained cyclooctynes (e.g. DBCO or BCN compounds) via Cu-free click reaction or with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. These reactions are bioorthogonal or in other words compatible with biological systems in that it′s components do not react with the biological environment.